Veranstaltung
| Titel | Endotheliale Regulation reproduktiver Organfunktionen |
| Title | Endothelial Regulation of Reproductive Organ Function |
| Schwerpunkt/Focus | |
| Sprache/Language | optional |
| VV-Nr./Course No. | 134365 |
| Modulverantwortlich/Responsible | Dr. Dorothee Bornhorst-Ohmstede |
| Vertreter/Co-responsible | |
| Anbieter/Teachers | Dr. Dorothee Bornhorst-Ohmstede |
| Typ/Type | Praktikum |
| SWS/Semerster periods per week | |
| Arbeitslast(h)/Work load | 300 h |
| KP/Credit points | 10 KP |
| Zuordnung/Classification | Forschungs-Modul |
| Semester/Semester | SoSe WiSe |
| Studierende/Students | MSc Biowissenschaften MSc Biotechnologie MSc Molekulare Biomedizin |
| Corona-Informationen/Corona-Information | Studierende müssen sich an unseren Hygieneplan halten. |
| Zeit/Date | 8 Wochen, nach Ankündigung |
| Ort/Location | ZMBE, Institut für Zellbiologie, Von-Esmarch-Str. 56 |
| Beginn/Start | nach Vereinbarung |
| Vorbesprechung/Obligatory pre-meeting | nach Vereinbarung |
| Voraussetzung/Prerequisite | 'keine' |
| Anmeldung/Registration | Über Dozentin |
| Leistungskontrollen/Performance assessments | Abschlusspräsentation |
| Termine f. Leistungskontrollen/Date for performance assessments | nach Vereinbarung |
| max. NP/Max. grade points | 200 |
| Ziele/Aims | The students will gain experience in endothelial and reproductive biology, zebrafish-based experimental techniques, molecular and cellular analysis, and the documentation of vascular and gonadal structures using microscopy. |
| Inhalte/Content | The biological context of the work is the role of blood and lymphatic endothelial cells in reproductive organ development and function, using zebrafish as an in vivo vertebrate model. The module includes experiments analyzing vascular–gonadal interactions and endothelial cell contributions to germ cell maturation and tissue homeostasis. Experimental approaches may include molecular manipulation of gene expression, fluorescence imaging of vascular and germ cell structures, and microscopy-based analysis of gonadal development and endothelial organization. |
| Methoden/Methods | The evaluation of gonadal and vascular phenotypes involves various techniques including confocal microscopy for high-resolution imaging of blood and lymphatic vessels, immunostaining of germ cells, live imaging in zebrafish, CRISPR/Cas9-based gene manipulation, targeted cell ablation approaches, and transcriptomic analyses to investigate endothelial–gonadal interactions during reproductive development. |
| Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills | |
| Voraussetzung für/Prerequisite for | |
| Präsenzpflicht/Compulsory presence | ja |
| Plätze/Number of participants | 1 |
| Gruppengröße/Group size | |
| Materialien/Materials | All materials are provided. |
| Literatur/Literature | The literature for this module will be discussed individually with the students during the preliminary meeting or at the beginning of the module. |
| Links | https://www.bornhorst-ohmstede-lab.de/ |
| Sonstiges/Further information |
Modulelemente:
Elemente of the module:
| Titel/Title | Zeit (von...bis)/Time (from...to) | Ort(Raum)/Location | |
|---|---|---|---|
| Übungen/Practical exercises | |||
| Vorlesung/Lecture | |||
| Seminare/Semeinars | |||
| Exkursionen/Excursions |
= Modul gehört zum SPP Imoplant / Module is part of the SSP Imoplant
= Modul gehört zum SPP Evolution /Module is part of the SSP Evolution
= Modul gehört zum SPP Bioanalytics and Biochemistry /Module is part of the SSP Bioanalytics and Biochemistry
= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
= Modul gehört zum SPP Quantitative Cell Biology /Module is part of the SSP Quantitative Cell Biology