| Titel |
Introduction to modern microscopy, molecular imaging and image analysis  
|
| Title |
Introduction to modern microscopy, molecular imaging and image analysis |
| Schwerpunkt/Focus |
ZPG |
| Sprache/Language |
englisch |
| VV-Nr./Course No. |
132227 |
| Modulverantwortlich/Responsible |
Prof. Dr. S. Luschnig |
| Vertreter/Co-responsible |
|
| Anbieter/Teachers |
S. Luschnig, I. Bedzhov, M. Bohnert, F. Kiefer, K. Schäfers, S. Hermann, A. Faust |
| Typ/Type |
Lecture, seminar and practical laboratory course |
| SWS/Semerster periods per week |
|
| Arbeitslast(h)/Work load |
150 h |
| KP/Credit points |
5 KP |
| Zuordnung/Classification |
Fortgeschrittenen-Modul (Biologie), Zusatzkompetenzmodul (Exp. Med.) Wahlpflichtfach (Medizin) |
| Semester/Semester |
WiSe |
| Studierende/Students |
MSc Biowissenschaften
MSc Biotechnologie
MSc Molekulare Biomedizin |
| Corona-Informationen/Corona-Information |
|
| Zeit/Date |
Block IV: 09.02. - 06.03.2026 |
| Ort/Location |
Multiscale Imaging Center MIC, Röntgenstrasse 16 |
| Beginn/Start |
09.02.2026, 10:00 |
| Vorbesprechung/Obligatory pre-meeting |
nach Vereinbarung |
| Voraussetzung/Prerequisite |
keine |
| Anmeldung/Registration |
online |
| Leistungskontrollen/Performance assessments |
Seminar presentation, poster presentation at the end of the practical laboratory course |
| Termine f. Leistungskontrollen/Date for performance assessments |
werden bei Vorbesprechung bekannt gegeben |
| max. NP/Max. grade points |
200 NP |
| Ziele/Aims |
The course aims to provide an overview of the spectrum of modern imaging modalities covering the scale from the individual cell to organs to complete organisms (yeast, Drosophila, mouse). A wide array of optical and preclinical imaging modalities will be introduced, including wide field and laser scanning microscopy, multiphoton microscopy, light sheet microscopy, MRI, CT and PET imaging. The different imaging modalities will be illustrated in the context of timely questions from the fields of developmental and cell biology and in animal models of widespread diseases. Background on the technical aspects of multiscale molecular imaging including the principles of signal and probe generation, probe pharmacology, and image reconstruction and evaluation methods are provided. |
| Inhalte/Content |
Physical and instrumental background of fluorescence, phosphorescence and advanced light microscopy are taught, providing the basis for a deeper understanding of epifluorescence, confocal, multiphoton and light sheet microscopy. Applying the aforementioned imaging modalities, structures and dynamic processes in cultured cells, embryos and tissue sections are visualized and reconstructed. High resolution imaging methods, like structured illumination, pixel reassignment and deconvolution are employed to identify and distinguish structures at or below the optical resolution limit. Methods for quantitative analysis of dynamic intracellular transport processes (FRAP) and protein interactions (FRET) are introduced. Fluorescent proteins and biosensors (GCaMP etc.) for measuring the dynamics of calcium-induced signaling processes are also introduced. The analysis of large tissue volumes and organisms bridges to the preclinical imaging modalities, MRI, CT and PET. Principles of nuclear and radiobiology, radiation and probe chemistry and pharmacodynamics are illustrated. An application example is the longitudinal detection of tumour growth. Various methods of image rendering, reconstruction and analysis, including Fiji, Omero, Voreen and Medgical, will be demonstrated and applied for the visual and statistical evaluation of generated data in planar and volume reconstructions. |
| Methoden/Methods |
Live cell imaging of genetically encoded sensors hypoxia sensors using widelfield fluorescence microscopy (time lapse microscopy, FRET/FLIM analysis). High resolution confocal imaging of subcellular structures and signals (cell cell junctions, receptor tyrosine kinases, calcium spikes) including the application of pixel reassignment methods for high resolution images and spectral unmixing to distinguish related fluorophores. Visualization of vascular structures in various organs by deep tissue imaging using multiphoton microscopy of light sheet imaging of optically cleared samples. The principles of wholemount tissue staining and sample preparation are introduced. Comparative longitudinal imaging of a tumor or inflammatory disease model in preclinical imaging is demonstrated. Image rendering and analysis approaches using public license / open access software, conceptualization, visualization and presentation of the generated data for the use in posters and talks. |
| Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills |
Image analysis and visualization software (e.g., Fiji, Imaris, Omero)
Conceptualization and visualization of data
Presentation techniques for effectively communicating research findings in poster and oral presentations" |
| Voraussetzung für/Prerequisite for |
keine |
| Präsenzpflicht/Compulsory presence |
ja/yes |
| Plätze/Number of participants |
10 |
| Gruppengröße/Group size |
|
| Materialien/Materials |
Werden gestellt. |
| Literatur/Literature |
Alberts et al.: Molecuar Biology of the Cell. Primärliteratur wird gestellt. |
| Links |
|
| Sonstiges/Further information |
|
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= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
= Modul gehört zum SPP Quantitative Cell Biology /Module is part of the SSP Quantitative Cell Biology